How to feed a worm: C. elegans as model in Molecular Nutrition and Physiology
The exploration of the interplay between the diet of an organism, its impact on gene and protein expression as well as on the metabolite profile is still at the scientific starting point. We use the model organism Caenorhabditis elegans to study the effects of a loss-of-function of the intestinal peptide transporter PEPT-1, a homologue of the mammalian PEPT1, on amino acid homeostasis, energy metabolism and lifespan. Coming from the site of diet composition, we explore the impact of flavonoid-enriched diets on stress resistance, development and/or ageing with main focus on selected signal transduction and metabolic pathways.
Useful C. elegans links:
Molecular biology: Cloning, PCR, RNA interference, GFP-reporter strains, DNA/RNA and protein extraction,
Microscopy: Light and fluorescence microscopy, confocal laser-scanning microscropy
Gene expression: Real-time RT-PCR (LightCycler), Microarray analysis (Affymetrix)
Protein expression: Western Blot, 2D-Gel-Electrophoresis, MALDI-TOF-MS, protein-protein interactions
Metabolic profiling: LC-MS/MS, amino acid analyser,
Other: uptake of radiolabeled amino acids, analysis of C. elegans development (brood size, generation time) and ageing (life span).
RNA interference (movie): http://www.nature.com/focus/rnai/animations/animation/animation.htm
last updated: 26.09.16, BS